The study of radiosensitivity in left handed compared to right handed healthy women
© Khosravifarsani et al.; licensee BioMed Central Ltd. 2012
Received: 7 February 2012
Accepted: 9 July 2012
Published: 24 August 2012
Radiosensitivity is an inheriting trait that mainly depends on genetic factors. it is well known in similar dose of ionizing radiation and identical biological characteristics 9–10 percent of normal population have higher radiation response. Some reports indicate that distribution of breast cancer, immune diseases including autoimmune diseases as example lupus, Myasthenia Gravies and even the rate of allergy are more frequent in left handed individuals compared to right handed individuals. The main goal of the present study is determination of radiosensitivity in left handed compared to right handed in healthy women by cytokinesis blocked micronuclei [CBMN] assay.
5 ml peripheral fresh blood sample was taken from 100 healthy women including 60 right handed and 40 left handed. The age of participants was between 20–25 old years and they had been matched by sex. After blood sampling, blood samples were divided to 2 groups including irradiated and non-irradiated lymphocytes that irradiated lymphocytes were exposed to 2 Gy CO-60 Gama rays source then chromosomal aberrations was analyzed by CBMN [Cytokinesis Blocked Micronuclei Assay].
Our results have shown radiosensitivity index [RI] in left-handers compared to right-handers is higher. Furthermore, the mean MN frequency is elevated in irradiated lymphocytes of left-handers in comparison with right-handers.
Our results from CBMN assay have shown radiosensitivity in the left handed is higher than right handed women but more attempts need to prove this hypothesis.
The Main goal of radiosensitivity recognition is radiation protection of radiation workers, identification of cancer patients which are radiosensitive to ionizing radiation after treating by radiotherapy and other treatment methods by ionizing radiation, identification of outcomes from atomic disasters and individualization of radiosensitivity in astronauts which are exposed to cosmic rays . In clinical radiotherapy, the patients that receive similar physical dose of ionizing radiation have different response to radiotherapy from latent to sever and sometimes lethal. Clinical radiotherapy of cancer patients have shown that 5–7 percent of cancer patients have adverse side effects in their normal tissues after clinical radiotherapy. These side effects are including late side effects, early side effects and cancer induction . Some reports have released that radiosensitivity is an inheriting trait in which genetic factors have a main role . The association of some SNPs and radiosensitivity has been described in several genes [1, 4–7]. Previous studies demonstrated that genes such as ATM and NBS which are involved in DNA repair mechanisms and cell cycle check point are responsible for hypersensitivity . Other studies described an association between genes such as LRRTM1  and PCSK6  and left handedness. It is well known that except genetic factors other parameters such as physical dose specifications or environmental conditions are important in radiosensitivity of cell and tissue. In similar specifications of physical dose and same environmental conditions it has been reported that some individuals have higher radiation response to ionizing radiations . Investigations on breast cancer distribution among postmenopausal women have also shown that left handed patients are approximately twice of right handed ones . Left handedness has been associated with some disorders such as thyroid and immune disorder especially autoimmune disease like lupus, multiple sclerosis, allergy, and migraine . All of these findings lead us to the study of radiosensitivity between the left- and right handed women by CBMN assay.
Materials and methods
Before bloods sampling all of participants filled patient consent form then 5 ml peripheral fresh blood sample was taken from 100 healthy women including 60 right handed and 40 left handed. Blood samples were then divided into two identical parts which one part was used as control-vehicle and the second part considered as exposed-vehicle. Environmental conditions for control and exposed vehicles were identical and blood sample transferring was close to ice pack. The age of participants was between 20–25 years and all of them were female. We used questionnaire about handedness of participants and their family. Handedness of participants was determined by oral questioning. History of cancer, previous irradiation, smoking, drug treatment and alcohol usage in participants and their family were considered as exclusion criteria.
Irradiation of peripheral blood samples was by 2 Gy of CO-60 gamma rays source (Theratone780 manufactured by Canada) and the dose rate of 120 CGy/min. Source to samples distance (SSD) was 80 cm and exposure area was 10 × 10 cm2. After irradiation, control and exposed blood samples were transferred for cell culture and were incubated in 37°C for 72 hr.
CBMN (Cytokinesis Blocked Micronuclei Assay)
CBMN assay was performed by IAEA protocol . 0.5 ml peripheral blood sample was added to 4.5 ml RPMI-1640 medium supplemented by 10% fetal calf serum, 1% Glutamine, 1% Penicillin/Streptomycin then 100 microliter Phytohaemagglutinin (PHA-M) was added to the medium. After 44 hr 100 microliter Cytochalasin B (6 μgr/ml diluted in 200 ml DMSO and 19.8 ml DW) was added to the medium. Cytochalasin B is an inhibitor of cytokinesis which prohibit from cell division in cytokinesis stage of cell cycle. 72 hr After PHA (SIGMA) addition, blood samples were harvested and then centrifuged with 2000 RPM for 10 min (BOECHO U-320 R). After which supernatant was wasted and 2–3 ml fresh hypotonic solution 0.075 M KCL was added and centrifuged with 1200 RPM for 7 min again then supernatant was wasted and 5 ml fixture solution containing methanol and acetic acid (6/1 methanol: glacial acetic acid) was added quickly. After 20 min the tubes were centrifuged (1200 RPM for 7 min) then supernatant was wasted. Fixation was repeated three times with similar procedure for good separation of binuclear lymphocytes. Afterward binuclear lymphocytes present in the pellet were dropped on cleaned and cooled slides. After 24 hr slides were stained by Giemsa (Diluted in PBS) 5% for 20 min then slides were washed by deionized water and air dried. All the slides were observed by light microscope in 40× magnification using SAIRAN microscope. All of the slides were coded before analyzing. Micronuclei were scored in 1000 binucleated (BN) cells and scoring was double blind. The micronuclei were scored according to the scoring criteria proposed by Fenech [14, 15]. The ratio of mean frequency of MN in exposed to non-exposed samples in each group was considered as radiosensitivity index .
Statistical analyze was performed by SPSS-16 software. Paired sample t-test was made between control and exposed group and independent sample t-test carried out between right handed and left handed subjects. P-Value ≤ 0.05 was considered as significance level.
Mean frequency of MN/ 1000 BN cells in right-handers and left-handers
We performed data analysis between irradiated and non_irradiated lymphocytes separately; our results released that mean frequency of MN observed in non-irradiated lymphocytes from right handed group compared to non-irradiated lymphocytes from left handed group is remarkably higher (P = 0.001). Furthermore the mean MN frequency observed in irradiated lymphocytes from right handed group compared to irradiated lymphocytes from left handed group is significantly elevated (P = 0.000).
Discussion and conclusion
Our results have shown that mean frequency of MN in irradiated lymphocytes is higher than non_irradiated lymphocytes in both right handed and left handed group. These findings are similar to former studies performed by Silva et al. , Koksal G et al.  and Gantonberg HW et al. . Our results have released that radiosensitivity index is elevated in left handed women compared to right handed women and also it's evident that difference of the mean MN frequency in left-handers compared to right-handers is higher. These findings are in agreement with another study reported by khosravifarsani et al. . Geschwind et al. have observed higher frequencies of immune disorders especially bowel, gut, learning disability, migraine and myasthenia gravis in left-handers and their relatives compared to right-handers .
The study of Made K Ramadhani et al. have shown that the distribution of breast cancer in postmenopausal women is more frequent in left handed compared to right handed women . Also Fritsch et al. showed that the risk of developing post-menopausal breast cancer in women is significantly higher in left-handers compared to right-handers . Stellman et al. showed a high association of left handedness with disease and disease risk factors . The study of Ashton  Coren and Halpern , Fleminger et al. , Kuhlemier , Lalumiere et al. , Lansky ; Porac et al.  and Tan, have revealed shortened life span in left handed individuals compared to right handed individuals . Investigations of Binali et al displayed that caries experience in left handed individuals have lower incidence than right handed individuals . S Geschwind and Galaburda have declared high proportion of mental retardation and developmental problems in left handed individuals . Former study demonstrated that the ABO blood groups are in relation with the radiation response in carcinoma of the cervix. On the basis of this study, O blood group has poorer response to radiotherapy than A and B merged and B blood group has lower response to ionizing radiation than A blood group .
Molecular and genome wide association studies are consistent with a polygenic model of handedness. Few genes have been suggested as candidates for the establishment of hand preference such as the imprinted gene LRRTM1 on 2p12-q11 , PCSK6 gene on 15q26.3  and the X-linked androgen receptor located on Xq11-12 [33, 34].
Although among few SNPs detected in left handed subjects none was studied as a risk factor for radiosensitivity, but in light of our findings one may hypothesis that those SNPs may either directly modify radiosensitivity by influencing DNA repair pathways or indirectly as part of a haplotype harboring a polymorphism involved in cell cycle control or DNA repair. In fact a recent molecular study indicates that carriers of MSH2 gIVS12-6nt-C allele show an increased radiosensitivity . MSH2 is one of the genes involved in DNA mismatch repair and located on chromosome 2 at 2p21. It has also been shown that polymorphism in Rad51 a gene located on chromosome 15 at 15q15.1 and involved in double strand break repair, can enhance cancer risk and in vitro chromosomal radiosensitivity [4, 5]. It has also been postulated that genetic polymorphism of androgen receptor may act as a risk-modifier for BRCA2-associated breast cancers .
Although our results have shown the association of left handedness and radiosensitivity, but to our knowledge no SNPs linked to DNA repair mechanisms or cell cycle check points have been studied in this regard. Further molecular investigations are needed to understand the possible genetic basis of this association.
The authors are grateful to staff of radiotherapy department of Shahid Rajae Hospital. Also the authors thank to staff of cellular and molecular biology research center of Babol University of Medical Sciences.
- Okaichi K, Ide M, Okumura Y: Radiation sensitivity and mutation position of p53. Int Congr Ser. 2003, 1258: 239-243.View ArticleGoogle Scholar
- Twardella D, Chang-Claude J: Studies on radiosensitivity from an epidemiological point of view – overview of methods and results. Radiother Oncol. 2002, 62: 249-260. 10.1016/S0167-8140(01)00491-1.View ArticlePubMedGoogle Scholar
- Popanda O, Ebbeler R, Twardella D, et al: Radiation-induced DNA damage and repair in lymphocytes from breast cancer patients and their correlation with acute skin reactions to radiotherapy. Radiation Oncology Biol Phys. 2003, 55 (5): 1216-1225. 10.1016/S0360-3016(02)04415-2.View ArticleGoogle Scholar
- Pratesi N, Mangoni M, Mancini I, Paiar F, Simi L, Livi L, Cassani S, Buglione M, Grisanti S, Almici C, Polli C, Saieva C, Magrini SM, Biti G, Pazzagli M, Orlando C: Association between single nucleotide polymorphisms in the XRCC1 and RAD51 genes and clinical radiosensitivity in head and neck cancer. Radiother Oncol. 2011, 99 (3): 356-361. 10.1016/j.radonc.2011.05.062.View ArticlePubMedGoogle Scholar
- Vral A, Willems P, Claes K, Poppe B, Perletti G, Thierens H: Combined effect of polymorphisms in Rad51 and Xrcc3 on breast cancer risk and chromosomal radiosensitivity. Mol Med Report. 2011, 4 (5): 901-912.Google Scholar
- Kelsey CR, Jackson L, Langdon S, Owzar K, Hubbs J, Vujaskovic Z, Das S, Marks LB: Polymorphism within the promoter of the TGFβ1 gene is associated with radiation sensitivity using an objective radiologic endpoint. Int J Radiat Oncol Biol Phys. 2012, 82 (2): e247-e255. 10.1016/j.ijrobp.2011.02.064.View ArticlePubMedGoogle Scholar
- Curwen GB, Murphy S, Tawn EJ, Winther JF, Boice JD: A study of DNA damage recognition and repair gene polymorphisms in relation to cancer predisposition and G2 chromosomal radiosensitivity. Environ Mol Mutagen. 2011, 52 (1): 72-76. 10.1002/em.20633.View ArticlePubMedPubMed CentralGoogle Scholar
- Jongmans W, Hall J: Cellular responses to radiation and risk of breast cancer. European journal of cancer (Oxford, England: 1990). 1999, 35 (4): 540-548. 10.1016/S0959-8049(98)00414-6.View ArticleGoogle Scholar
- Francks C, Maegawa S, Lauren J, Abrahams BS, Velayos-Baeza A, Medland SE, et al: LRRTM1 on chromosome 2p12 is a maternally suppressed gene that is associated paternally with handedness and schizophrenia. Molecular psychiatry. 2007, 12 (12): 1129-1139. 10.1038/sj.mp.4002053. 057View ArticlePubMedPubMed CentralGoogle Scholar
- Scerri TS, Brandler WM, Paracchini S, Morris AP, Ring SM, Richardson AJ, et al: PCSK6 is associated with handedness in individuals with dyslexia. Hum Mol Genet. 2011, 20 (3): 608-614. 10.1093/hmg/ddq475.View ArticlePubMedGoogle Scholar
- Ramadhani MK, Elias SG, van Noord PA, Grobbee DE, Peeters PH, Uiterwaal CS: Innate left handedness and risk of breast cancer: case-cohort study. BMJ. 2005, 331 (7521): 882-883. 10.1136/bmj.38572.440359.AE.View ArticlePubMedPubMed CentralGoogle Scholar
- Geschwind N, Behan P: Left-handedness: association with immune disease, migraine, and developmental learning disorder. Proc Natl Acad Sci U S A. 1982, 79 (16): 5097-5100. 10.1073/pnas.79.16.5097.View ArticlePubMedPubMed CentralGoogle Scholar
- Agency IAE: Cytogenetic Analysis For Dose Assessment. Technical Report series No 405. 2001, Vienna: IAEAGoogle Scholar
- Fenech M: The in vitro micronucleus technique. Mutat Res. 2000, 455: 81-95. 10.1016/S0027-5107(00)00065-8.View ArticlePubMedGoogle Scholar
- Fenech MF: The cytokinesis-block micronucleus technique. Technologies for Detection of DNA Damage and Mutations. Edited by: Pfeifer GP. 1996, New York: Plenum Press, 25-36.View ArticleGoogle Scholar
- Khosravifarsani M, Monfared A, Elahimanesh F, Borzoueisileh S, Hajian-Tilaki K, Seyfizadeh N, et al: Is there association between handedness and radiosensitivity in breast cancer women?. Medical Oncology. 1-4.
- Silva MJ, Carothers A, Dias A, et al: Dose dependence of radiation induced micronuclei in cytokinesis blocked human lymphocytes. Mutation Res. 1994, 322: 117-128. 10.1016/0165-1218(94)00019-0.View ArticlePubMedGoogle Scholar
- Dalci DO, Pala FS: Micronuclei in human lymphocytes. The Co-60 gamma ray dose response. Mutation Res. 1996, 359: 151-157. 10.1016/S0165-1161(96)90261-7.View ArticlePubMedGoogle Scholar
- Gantonberg HW, Wuttke K, Streffer C, et al: Micronuclei in human lymphocytes irradiated in vitro or in vivo. Radiation Res. 1991, 128: 276-281. 10.2307/3578050.View ArticleGoogle Scholar
- Fritschi L, Divitini M, Talbot-Smith A, Knuiman M: Left-handedness and risk of breast cancer. Br J Cancer. 2007, 97 (5): 686-687. 10.1038/sj.bjc.6603920.View ArticlePubMedPubMed CentralGoogle Scholar
- Stellman S, Wynder EL, De Rose DJ, Muscat JE: The epidemiology of left handedness in a hospital. Ann Epidemiol. 1997, 7: 167-171. 10.1016/S1047-2797(96)00158-5.View ArticlePubMedGoogle Scholar
- Ashton GC: Handedness: an alternative hypothesis. Behav Genet. 1981, 12: 125-147.View ArticleGoogle Scholar
- SaDFH C: Left handedness: a marker for decreased survival fitness. Psychol Bull. 1991, 109: 90-106.View ArticleGoogle Scholar
- Fleminger JJ, Dalton R, Standage KJ: Age as a factor in the handedness of adults. Neuropsychologia. 1977, 15: 15-View ArticleGoogle Scholar
- Kuhlemeier KV: Longevity and left handedness. Am J Public Heath. 1991, 81: 513-View ArticleGoogle Scholar
- Lalumière ML, Blanchard R, Zucker KJ: Sexual orientation and handedness in Men and Women: a meta-analysis. Psychol Bull. 2000, 126: 575-592.View ArticlePubMedGoogle Scholar
- Lansky LM, Feinstein H, Peterson JM: Demography of handedness in two samples of randomly selected adults. Neuropsychology. 1988, 26: 465-477. 10.1016/0028-3932(88)90099-1.View ArticleGoogle Scholar
- Porac C, Coren S, Duncan P: Lateral preference in retardates: Relationships between eye, foot and ear preference. J Clin Neuropsychol. 1980, 2: 173-187. 10.1080/01688638008403791.View ArticleGoogle Scholar
- Tan LE: Handedness in two generations. Percept Mot Skills. 1983, 56: 867-874. 10.2466/pms.1918.104.22.1687.View ArticlePubMedGoogle Scholar
- Cakur B, Yildiz M, Dane S, Zorba YO: The effect of right or left handedness on caries experience and oral hygiene. J Neurosciences in Rural Practice. 2011, 2 (1): 40-42. 10.4103/0976-3147.80093.View ArticleGoogle Scholar
- Geschwind N, Galaburda AM: Cerebral Lateralization: Biological mechanisms, associations and pathology. I-III. Arch Neurol. 1985, 42: 428-458. 10.1001/archneur.1985.04060050026008. 521–52, 634–54View ArticlePubMedGoogle Scholar
- Garriga R, Ghossein NA: The ABO blood groups and their relation to the radiation response in carcinoma of the cervix. Cancer. 1963, 16: 170-172. 10.1002/1097-0142(196302)16:2<170::AID-CNCR2820160205>3.0.CO;2-X.View ArticlePubMedGoogle Scholar
- Medland SE, Duffy DL, Spurdle AB, Wright MJ, Geffen GM, Montgomery GW, Martin NG: Opposite effects of androgen receptor CAG repeat length on increased risk of left handedness in males and females. Behav Genet. 2005, 35: 735-744. 10.1007/s10519-005-6187-3.View ArticlePubMedGoogle Scholar
- Hampson E, Sankar JS: Hand preference in humans is associated with testosterone levels and androgen receptor gene polymorphism. Neuropsychologia. 2012, 50 (8): 2018-2025. 10.1016/j.neuropsychologia.2012.04.027.View ArticlePubMedGoogle Scholar
- Mangoni M, Bisanzi S, Carozzi F, Sani C, Biti G, Livi L, Barletta E, Costantini AS, Gorini G: Association between genetic polymorphisms in the XRCC1, XRCC3, XPD, GSTM1, GSTT1, MSH2, MLH1, MSH3, and MGMT genes and radiosensitivity in breast cancer patients. Int J Radiat Oncol Biol Phys. 2011, 81 (1): 52-58. 10.1016/j.ijrobp.2010.04.023.View ArticlePubMedGoogle Scholar
- Shin S, Verma IM: BRCA2 cooperates with histone acetyltransferases in androgen receptor-mediated transcription. Proc Natl Acad Sci USA. 2003, 100 (12): 7201-7206. 10.1073/pnas.1132020100.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1756-6649/12/3/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.