Microwave open-ended coaxial dielectric probe: interpretation of the sensing volume re-visited
© Meaney et al.; licensee BioMed Central Ltd. 2014
Received: 21 September 2013
Accepted: 11 June 2014
Published: 17 June 2014
Tissue dielectric properties are specific to physiological changes and consequently have been pursued as imaging biomarkers of cancer and other pathological disorders. However, a recent study (Phys Med Biol 52:2637–2656, 2007; Phys Med Biol 52:6093–6115, 2007), which utilized open-ended dielectric probing techniques and a previously established sensing volume, reported that the dielectric property contrast may only be 10% or less between breast cancer and normal fibroglandular tissue whereas earlier data suggested ratios of 4:1 and higher may exist. Questions about the sensing volume of this probe relative to the amount of tissue interrogated raise the distinct possibility that the conclusions drawn from that study may have been over interpreted.
We performed open-ended dielectric probe measurements in two-layer compositions consisting of a background liquid and a planar piece of Teflon that was translated to predetermined distances away from the probe tip to assess the degree to which the probe produced property estimates representative of the compositional averages of the dielectric properties of the two materials resident within a small sensing volume around the tip of the probe.
When Teflon was in contact with the probe, the measured properties were essentially those of pure Teflon whereas the properties were nearly identical to those of the intervening liquid when the Teflon was located more than 2 mm from the probe tip. However, when the Teflon was moved closer to the probe tip, the dielectric property measurements were not linearly related to the compositional fraction of the two materials, but reflected nearly 50% of those of the intervening liquid at separation distances as small as 0.2 mm, and approximately 90% of the liquid when the Teflon was located 0.5 mm from the probe tip.
These results suggest that the measurement methods reported in the most recent breast tissue dielectric property study are not likely to return the compositional averages of the breast tissue specimens evaluated, and thus, the conclusions reached about the expected dielectric property contrast in breast cancer from this specimen study may not be correct.
Tissue dielectric properties have long been of interest to researchers because of their significant differences between tissue types [1–3]. Scientists have speculated that these properties could be harnessed for their potential to detect cancers because tumors are generally considered to have elevated water content when compared to normal tissue because of the increased hydration associated with the rapid metabolism of cancer cells and the surrounding angiogenic vasculature [4, 5]. Breast cancer detection has been considered as a particularly good opportunity because the surrounding normal breast tissue of most women is dominated by adipose tissue which is well known to have low water content, and concomitantly low dielectric properties [6, 7]. Early studies confirmed these expectations [7–9] but variations in the results raised questions about the data in each report. For instance, the especially low permittivity values reported in the Chaudhary study suggest that the measured tissue specimens were primarily composed of fat, and overlooked the dielectric property contributions from the normal fibroglandular breast parenchyma. A much larger and more comprehensive investigation by the Universities of Wisconsin and Calgary [10, 11] assessed breast cancer contrast levels more systematically with state-of-the-art open-ended coaxial probes, and correlated the data with co-registered histopathological analyses that accounted for important contributions from factors which included the integrated fibroglandular tissue fraction. These more recent results indicate that the dielectric property contrast for breast cancer relative to a background of normal fibroglandular tissue is only a fraction of the ratios reported in previous studies. Not surprisingly, this study has influenced the direction of microwave breast imaging research, and has steered investigations towards the development of systems requiring external contrast agents [12, 13] despite the fact that in vivo clinical studies are emerging which demonstrate cancer detection and monitoring to statistically significant diagnostic accuracies based on endogenous dielectric property contrast in the breast [14–17].
The development of open-ended coaxial dielectric probes during the 1980s and 1990s facilitated the routine measurement of high frequency (i.e. >100 MHz) tissue dielectric properties that were often obtained from ex-vivo specimens in the case of human tissues (because of convenience/access) [8, 9, 18–26], although in-vivo data from animal studies were also commonly reported [27, 28]. These instruments are generally considered to be the gold standard or to provide the ground truth when characterizing a tissue’s electromagnetic properties because the tools can be validated against homogeneous samples of materials with (already) known dielectric properties. However, the sampling volume of dielectric probes, and especially how the signals (and their subsequent conversion into dielectric property estimates) from that sampling volume are influenced by small-scale property heterogeneity is critical in tissues (few are homogeneous or even reasonable approximations to the homogeneous media utilized in probe validation studies, for example, non-fatty breast tissue consists of variable patterns and percentages of interwoven adipose and fibroglandular compositions [6, 9]), but is rarely considered in detail.
Hagl et al.  did investigate the sampling volume question and found a sensing volume of 1.5 mm (in depth) by 5 mm (in width) for a 2.2 mm diameter open-ended coaxial probe with an approach that was subsequently used to determine a sensing volume of 3 mm (in depth) by 7 mm (in width) for a 3 mm diameter dielectric probe which was applied in two large breast tissue specimen studies [10, 11]. However, the experiments considered by Hagl et al. were based on homogeneous liquids in which probe tips were systematically moved to positions close to the base and side walls of a glass beaker to infer their concomitant sampling volumes (by assuming the probe’s sampling volume corresponded to the minimum volume of liquid that existed before the first evidence of signal change occurred sufficient to alter the dielectric property estimates). Unfortunately, these experiments find the minimum volume of a homogeneous liquid that is needed to measure its dielectric properties accurately, but do not determine the probe’s sampling volume, or more importantly, how the probe’s signals from the said sampling volume are influenced when the properties are not actually homogeneous. For example, if the Hagl experiments performed do approximate the sampling volume of the probe, then presumably the resultant probe property estimates from a heterogeneous sample would represent an effective average of the compositional percentages of those materials contained within the probe’s sampling volume.
In this paper, we present data from several simple experiments similar to those performed by , in which layered properties are used to investigate the influence of heterogeneity on the probe’s dielectric property estimates when the layers are in close proximity to the tip of the probe. While layered structures offer only one class of the infinite number of heterogeneous property distributions that exist or could be considered, they are easily controlled and simplify the problem by eliminating effects from heterogeneity in the lateral directions. The results, unfortunately, suggest that the dielectric properties are disproportionately influenced by the material resident within the first 200–400 microns of distance from the probe tip, and the probe does not behave as having a much larger sensing volume in which the resultant dielectric property estimates represent a compositional average of the dielectric properties of the materials within the volume. Numerical simulations confirm consistency between model and measurement. Even more unfortunate are the implications of these results on interpretations being made and conclusions being drawn from the data reported in [10, 11]. These widely cited studies are often considered to be the definitive data on the electromagnetic properties of breast tissue/tumor, and while they do represent the largest and most systematic effort completed to date to probe the dielectric properties of breast surgical specimens, the results presented here suggest that those measurements are surface-property biased, and likely do not represent the effective dielectric properties of the volume averaged tissue that could, for example, be recovered on a cm-scale through non-invasive microwave imaging methods [14, 15, 17].
Dielectric probe measurement tank
Dielectric property calculations
We used the standard Agilent Dielectric Probe Kit (85070E) and the associated software package to compute the dielectric properties over a prescribed frequency range . We have validated the technique in numerous different experiments. This technique is considered accurate to within 1 and 3% for the real and imaginary permittivity values, respectively, over the range of 1–12.5 GHz  and increase progressively for frequencies extending to 50 GHz. The measurements were calibrated with the standard procedure – in this case based on recordings from an open circuit (air), a short circuit and de-ionized water. The software essentially infers from the S-parameter data (in this case S11) the dielectric properties that are required to generate the associated reflection measurement. For these experiments, the shorting configuration supplied by the vendor was not convenient because of the size of the tank. Instead, a piece of aluminum foil with a soft rubber backing was used to ensure intimate contact with the probe. Benchmark measurements of Teflon, air and water were made to confirm that the probe was calibrated correctly and operating properly.
Simulations of the fields near the probe for different measurement configurations were performed with CST Microwave Studio software (Framingham, MA) at 2 GHz and were intended to illustrate the impact of different materials on the field patterns which ultimately influence the dielectric property calculations. Dimensions of the probe and the borosilicate glass bead at the end of the open-ended coax were taken from Blackham and Pollard . S11 values for the coaxial probe placed against a layered medium were computed through model analyses developed by Hodgetts  and tested in experiment by Gregory et al. . This approach finds the electric fields in a geometry in which samples are bounded by a conducting cylinder. For the data presented, the size of the cylinder was chosen so that the presence of the conducting cylinder has negligible effect. It has been validated against other published methods with an error of less than 0.1%. The inversion technique to recover the complex permittivity from the S11 data utilized a gradient-descent method with first order differentiation developed by Grant et al. . It has been validated with respect to probe dimensions over a range of dielectric properties and associated frequencies.
The data presented in this paper confirm the findings in Hagl et al.  which indicate a certain volume of material is required to achieve accurate measurements of a homogeneous sample. However, results presented here are the first to consider the dielectric property estimates obtained from an open-ended coaxial dielectric probe over microwave frequencies in the presence of a heterogeneous medium under controlled experimental conditions. Specifically, a layered volume with highly contrasting electrical properties was evaluated, and the probe recordings were consistent independently of whether the intervening layer was water or the more lossy (especially at lower frequencies) saline. The recovered properties reached 50% and 90% of those of the intervening liquid even when the fluid layer was only 0.2 and 0.5 mm thick, respectively. While the material (Teflon) at distance (from the probe) beyond the liquid layer exerted some influence, its effect was significantly diminished relative to the liquid immediately adjacent to the probe surface.
One of the challenges in deploying dielectric probes is the maintenance of contact between the probe and the material under test. Thus, they are ideal for liquid testing and soft tissue measurements because both naturally conform to the surface of the open-ended coaxial line. Accordingly, vendors such as Agilent Technologies (85070E Dielectric Probe Kit) do not recommend these probes for measuring the dielectric properties of hard materials.
The implications of this report are potentially profound because the long-held presumption that an open-ended dielectric probe provides an accurate estimate of tissue properties over a heterogeneous sensing volume 2 to 3 mm below the surface of the probe is not likely to be correct. While 2–3 mm may appear to be a relatively small distance over which the dielectric probe might reasonably be expected to provide accurate property estimates, the reality is that the material within the first few hundred microns exerts the dominant influence on the estimated properties. If the open-ended coaxial probe does not recover an appropriately averaged property estimate in the layered test configuration considered here, its fidelity when used to measure the dielectric properties of more randomly arranged heterogeneous mixtures of tissue is questionable – a finding that raises serious questions about how best to utilize these probes when measuring the properties of tissues that are as heterogeneous as the breast which commonly has infiltrations of fibroglandular tissue interwoven within a matrix of adipose cells . Because adipose tissue is more predominant in the breasts of many women, it is largely homogeneous and easily sampled. Fibroglandular breast tissue is more challenging, and in this respect, the results of Joines et al.  are particularly revealing because these investigators did attempt to separate mammary from the adipose tissues. While the Joines results only considered the frequency range from 50 to 900 MHz, their data are unambiguous in terms of demonstrating a large dielectric property contrast between malignant and mammary tissue – as much as 4:1 and 7:1 for permittivity and conductivity, respectively. These findings are in stark contrast with those of the Lazebnik et al. reports [10, 11] which indicate a much smaller contrast (~10%) between malignant and fibroglandular breast tissues. While the methodology used in the Lazebnik et al. reports is sound, the results presented here indicate that the data are very likely less conclusive than is suggested in subsequent literature, and some caution is advised when interpreting these results as the basis for determining whether bulk tissue contrast (on the cm-scale) exists in the electromagnetic properties of normal versus abnormal breast tissues. Indeed, the dielectric probes used in the Lazebnik studies are not likely to return the compositional averages of the tissue specimens evaluated, and thus, the conclusions reached in the study about the expected dielectric property contrast in breast cancer may not be accurate. Accordingly, over-interpreting these results could have unintended consequences, for example, in unnecessarily steering the microwave breast imaging research community away from imaging methods based on endogenous breast tissue dielectric contrast for cancer detection. In light of the sensing volume nonlinearities of open-ended dielectric probes identified in this paper, especially when considered in the context of the positive clinical microwave breast imaging results that are emerging, caution is recommended when concluding that substantial microwave property contrast does not exist in breast cancer.
Scattering matrix parameter
Scattering matrix parameter for a reflected signal at port 1 relative to an incident signal at port 1
We would like to thank B. G. Loader at the National Physical Laboratory for assistance in using CST Microwave Studio.
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