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Comparison of Technetium-99m-MIBI imaging with MRI for detection of spine involvement in patients with multiple myeloma



Recently, radiopharmaceutical scanning with Tc-99m-MIBI was reported to depict areas with active bone disease in multiple myeloma (MM) with both high sensitivity and specificity. This observation was explained by the uptake of Tc-99m-MIBI by neoplastic cells. The present investigation evaluates whether Tc-99m-MIBI imaging and magnetic resonance imaging (MRI) perform equally well in detecting myelomatous bone marrow lesions.


In 21 patients with MM, MRIs of the vertebral region TH12 to S1 and whole body scans with Tc-99m-MIBI were done.


Tc-99m-MIBI scanning missed bone marrow infiltration in 43 of 87 vertebrae (50.5%) in which MRI showed neoplastic bone marrow involvement. In patients with disease stage I+II, Tc-99m-MIBI scanning was negative in all of 24 vertebrae infiltrated according to MRI. In patients with disease stage III, Tc-99m-MIBI scanning detected 44 of 63 (70%) vertebrae involved by neoplastic disease.


Tc-99m-MIBI scanning underestimated the extent of myelomatous bone marrow infiltration in the spine, especially in patients with low disease stage.

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The leading symptom of multiple myeloma (MM) is neoplastic bone involvement. Difficulties in diagnosing MM can arise from the fact that not all patients present with punched-out osteolytic lesions, the typical radiographic findings of MM. In 10–20% of patients with first diagnosis of MM, the skeletal x-rays are completely normal, and in up to 10% only osteoporosis-like changes can be detected. In contrast to skeletal radiography, which reveals the osseous destruction induced by myeloma cells, magnetic resonance imaging (MRI) directly depicts the initiator of bone lesions, myelomatous bone marrow infiltration [1]. Since bone marrow involvement can be visualized by MRI, even when the lytic lesions cannot be seen on skeletal radiographs, MRI has markedly improved the diagnosis and monitoring of MM [1]. Recently, radiopharmaceutical scanning with Technetium-99m 2 methoxy-isobutyl-isonitrile (Tc-99m-MIBI) was shown to demonstrate areas with active bone disease in MM with both high sensitivity and specificity [26]. Fonti et al. reported that myeloma cells directly take up Tc-99m-MIBI in vitro and that there is a close correlation between both the in vitro and in vivo uptake of the radiolabeled tracer and the bone marrow plasma cell infiltration shown by bone marrow aspiration [6]. Thus, Tc-99m-MIBI scanning reveals the presence of the infiltrating myeloma cells rather than its consequence, neoplastic bone destruction. However, in the studies published yet, the results of Tc-99m-MIBI scanning have been correlated with plain skeletal radiographs and/or with bone scintigraphy, both examination methods which reveal the neoplastic bone destruction but not the bone marrow infiltration (as does MRI). The aim of the present investigation was to evaluate whether MRI and Tc-99m-MIBI scanning perform equally well in detecting myelomatous bone marrow lesions in the spine as an often affected site.

Patients and methods

Twenty-one consecutive patients with MM (15 females, 6 males; median age 68 years, range: 39 – 88 years) participated in the study. The diagnosis of MM and the staging of disease were based on standard criteria [7]. Four patients had disease stage I, 5 disease stage II and 12 disease stage III. Seventeen patients were entered into the study at first diagnosis of MM (prior to the first administration of chemotherapy or radiotherapy), 4 patients at disease progression. None of the patients had been irradiated in areas of the spine evaluated for myelomatous bone marrow infiltration by MRI or Tc-99m-MIBI scanning.

All patients underwent MRI of vertebrae TH12, L1, L2, L3, L4, L5 and S1. MRI was performed using a 1.0 Tesla Magneton Expert (Siemens) with a spinal coil. Sagittal images included a T1-weighted spin echo sequence and a fat-suppressed T2-weighted fast spin echo sequence. If a finding was ambiguous, the T1-weighted images were repeated after the intravenous injection of gadolinium chelate. All images were classified into two categories: normal findings and myelomatous involvement (focal, diffuse or heterogenous pattern) [1].

Anterior and posterior whole body scans (8 min for each projection) were obtained ten minutes, 1 hour, 4 hours and (in four randomly selected patients) 24 hours after the intravenous injection of 555 MBq of Tc-99m-MIBI. A large field of view (LFOV) double head gamma camera (Elscint Helix; Haifa) equipped with a low-energy general purpose collimator was used. Additionally, one hour after tracer application, a posterior spot view of the lumbar vertebral column and pelvic area was performed using a low-energy high resolution collimator. The scans were classified as showing [3]:

- a normal pattern with only physiological uptake (i.e., heart, liver and spleen),

- a pathologic pattern, with areas of focal tracer uptake and/or diffuse bone marrow uptake.

The two investigators (evaluating either the magnetic resonance images or the images obtained by Tc-99m-MIBI scanning) were blinded with regard to tumor stage and to the findings by the opposite imaging method.

If MRI or Tc-99m-MIBI scanning revealed several pathologic areas in one and the same vertebra of an individual patient, these were counted as one positive finding for the respective examination method.

Mononuclear cells isolated from bone marrow aspirates were available in 16 patients. These cells were stained with two different monoclonal antibodies that recognize different epitopes of the P-glycoprotein in order to evaluate the multi drug resistance as a possible reason for missing Tc-99m-MIBI uptake [8].

The study was performed in accordance with the Declaration of Helsinki, amended by the 29th (Tokyo) and 35th (Venice) World Medical Assembly, and in accordance with the pertinent national laws. The protocol was approved by the local ethical committees. Patients gave their informed consent prior to enrollment in the study.


In the 21 patients, a total number of 147 vertebrae were examined. All the lesions found by Tc-99m-MIBI scanning were already detectable one hour after the administration of the radiolabelled tracer. The four-hour and 24-hour examinations did not further improve the results of the Tc-99m-MIBI scanning. In the entire collective, Tc-99m-MIBI scanning missed myelomatous bone marrow infiltration in 43 of 87 vertebrae (50.5%) in which MRI showed neoplastic bone marrow involvement (Table 1). In four patients, Tc-99m-MIBI scanning identified none of the vertebrae infiltrated according to MRI. Categorizing patients according to tumor stage revealed that in patients with disease stage I+II Tc-99m-MIBI scanning was negative in all of 24 vertebrae in which myelomatous infiltration was shown by MRI. In patients with disease stage III, Tc-99m-MIBI scanning detected 44 of 63 (70%) involved vertebrae. Therefore, the proportion of involved vertebrae detected by Tc-99m-MIBI scanning was significantly (chi-square test: p < 0.002) higher in patients with disease stage III than in patients with disease stage I+II. Tc-99m-MIBI scanning was positive in only 4 vertebrae (of two patients with disease stage I) in which MRI showed a normal structure. In a follow-up of these 2 untreated patients two years later, examination by MRI still yielded no signs of neoplastic infiltration in these 4 vertebrae.

Table 1 Relationship of positive and negative findings in MRI and Tc-99m-MIBI scanning

Discussion and Conclusion

In comparison with magnetic resonance imaging, Tc-99m-MIBI scanning grossly underestimated the extent of myelomatous bone marrow infiltration, especially in patients with low disease stage. Since MM is a disease most often characterized by focal and not diffus neoplastic bone marrow infiltration, random bone marrow sampling may not be entirely diagnostic or predictive of disease status [7]. Thus, the observation of Fonti et al. who showed a significant correlation between the intensity of Tc-99m-MIBI uptake and plasma cell density in bone marrow aspirates should be considered with caution. In the majority of MM patients whose bone marrow is not diffusely infiltrated by neoplastic cells, MRI provides a better assessment of tumor load, than does single bone marrow aspiration [9]. However, the advantage of Tc-99m-MIBI scintigraphy is the image of whole body in one single examination and it is less time consuming than MRI and therefore more costeffective and comfortable for the patient.

According to Tirovola et al. [4], the false negative results of Tc-99m-MIBI scanning might be due to the presence of the multidrug resistance associated P-glycoprotein, which accelerates the efflux of Tc-99m-MIBI (and of several cytostatics) from MM cells. In our study, we tested for the presence of the P-glyocoprotein by means of immunocytochemistry. In the 16 patients examined (including three of the four patients with totally negative Tc-99m-MIBI scans but with lesions detected by MRI) no evidence of P-glycoprotein expression was found.

Further it must be taken into consideration, that a mild activity of 99mTc-Tc-99m-MIBI can be observed even in normal bone and bone marrow [10]. Thus, further prospective studies with Tc-99m-MIBI seem to be necessary in order to define a reliable scintigraphic pattern, i.e. relative index to background or to an not affected skeletal area, for suspected parts of the skeleton as the spine, at which a site comparison is not possible as for the extremities.


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We thank Ms. Jane Neuda for editorial assistance.

The study was supported by a grant from "Oesterreichisches Forum gegen Krebs".

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Correspondence to Martin Pecherstorfer.

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None declared.

Authors' contributions

MP and HL designed the study and drafted the manuscript.

SM and HK carried out the nuclear medicine investigation.

PK was responsible for quality control of nuclear medicine investigation.

WM made the MRI analysis.

SM and AK performed the correlation of scintigraphic and MRI findings and participated in drafting the manuscript.

MF performed the immunochemistry examinations.

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Mirzaei, S., Filipits, M., Keck, A. et al. Comparison of Technetium-99m-MIBI imaging with MRI for detection of spine involvement in patients with multiple myeloma. BMC Med Phys 3, 2 (2003).

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  • Multiple Myeloma
  • Bone Marrow Infiltration
  • Bone Marrow Uptake
  • Diffuse Bone Marrow
  • Skeletal Radiograph